Estudo da variabilidade genética e dos fatores de virulência de isolados de Ureaplasma diversum. / Study of genetic variability and virulence factors of Ureaplasma diversum isolates.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The aim of the present study was the study of genetic variability and virulence factors of U. diversum clinical isolates. The strains were submitted to sequencing for 16S rRNA and urease genes. Moreover, the strains were analyzed to the virulence factors: capsule, phospholipase C, IgA protease and adhesion and invasion into Hep-2 cells. The sequencing of parcial 16S rRNA gene showed polymorphic patterns into 44 positions. These polymorphisms clustered the strains in seven groups. For the virulence factors, ureaplasma cells showed a dense-stained external capsule-like structure surrounding the cell membrane. A high level of phospholipase C activity was also detected in 31 studied ureaplasma. However, no strains showed IgA protease activity. For the invasion assay, the isolates and strains used were detected inside the cells after infection of one minute. The invasions of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% to 10% of studied ureaplasmas were inside the infected cells.

ASSUNTO(S)

immunoglobulins variação genética bacterial invasion cápsulas imunoglobulinas phospholipases c microbiologia microbiology bactérias aeróbicas gram-negativas iga protease invasão bacteriana aerobic gram-negative genetic variation ureaplasma diversum fosfolipases c capsules ureaplasma diversum ig a protease

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