Estudo da proliferação celular pelo anticorpo anti-PCNA e tecnica AgNOR em biopsias ensoscopicas da mucosa gastrica

AUTOR(ES)

Silvia Pierre Irazusta

DATA DE PUBLICAÇÃO

1994

RESUMO

The proliferation cell nuclear antigen (PCNA) is a 36 Kda protein, acting as a synergist of the delta-DNA-polymerase. As compared to other methods to study cell proliferation, its detection by immuno-histochemistry using a monoclonal antibody to PCNA, PC 10, has the advantage of being simple and preserves tissue morphology as well. It can be performed in formalin-fixed, paraffin embedded tissues, allowing studies in archival material. Such advantages are shared with the detectation of nucleolar organizing regions by a silver impregnation technique (AgNOR). The number of black spots, which reflect the silver affinity of proteins related to NORs, has been correlated to cell proliferation. The aim of this retrospective study is to verify to which extent the rate of PC 10 - positive - cells and quantification of AgNORs can be helpful in identifying normal, inflammatory/regenerative, dysplastic and neoplastic gastric mucosa. The results obtained by both methods in 57 endoscopic pyloric biopsies reveal significant higher counts in the neoplastic group, as compared to other conditions (p <0,001). Nevertheless, distinction of each group by quantification of PC 10 and AgNORs alone is not possible, due to overlap of values. This represents a handicap of both methods for diagnosis in individual cases. Regression analysis shows a statistically significant correlation between both methods only in the normal and inflammatory/regenerative groups. For the other conditions there is no statistical significance, possibly because proteins detected by both methods are diversely expressed and regulated during the cell cycle. Although AgNOR quantification is of limited diagnostic value, irregular shaped spots can be considered reliable markers for malignancy

ASSUNTO(S)

região organizadora do nucleolo celulas - proliferação mucosa gastrica

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