Estudo da capacidade antioxidante total em tecidos e fluidos biologicos usando a voltametria de pulso diferencial / Total antioxidant capacity in tissues and body fluids: a differential pulse voltammetry study

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The objective of this study was to analyze the changes in total antioxidant capacity conferred by low molecular weight antioxidants (LMWA) in biological fluids and tissues by the use of differential pulse voltammetry (DPV). Chapter 1 is a review article about the possible fonts of reactive oxygen species generation in skeletal muscle during exerci se. Chapter 2 is a divulgation article that deals with antioxidant defense systems, LMWA detection methods, cyclic voltametry and DPV. The paper in chapter 3 presents the results about LMWA concentration in plasma and saliva of athletes submitted to physical training. It was observed a decrease in plasma total antioxidant capacity after 6 weeks of training (879.28 :t 197.92 !-Imol Trolox equiv. L-1 in second week versus 787.82:t 154.71 !-Imol Trolox equiv. L-1 in seventh week) associated to an enhanced physical performance in specific tests and maintained indices of muscle damage and inflammation. The data suggests that a small decrease in the plasma antioxidant capacity is part of a normal answer in the initial stages of a physical training programo It was also shown in this study a significant correlation between plasma and saliva antioxidant capacity (r=0.5871, p<0.01) and a significant correlation between this samples antioxidant capacity and their respective urate concentrations. In chapter 4 the kinetics of liver and skeletal muscle hydrophilic and lipophilic LMWA and enzymatic antioxidants from rats submitted to exhaustive exerci se preceded by 24h of fasting, are shown. Liver and muscle hydrophilic extractions and liver lipophilic extraction presented two classes of LMWA while muscle lipophilic extraction presented only one class. It was observed a significant decrease in the antioxidant capacity conferred by hydrophilic and lipophilic LMWA and enzymatic antioxidants in ali analyzed moments (30 min, 1, 6 e 24 h). Only one class of muscle hydrophilic LMWA showed to be reestablished 24h after exerci se. Liver lipophilic first and second classes of LMWA were increased in 57% and 42% respectively after fasting. Enzymatic antioxidants activities were also reduced during the whole analyzed period, presenting a strong correlation with the antioxidant capacity conferred by LMWA. These is the first report of the use of differential pulse voltammetry for total antioxidant capacity determination conferred by LMWA of biological fluids as plasma and saliva and tissues like liver and skeletal muscle

ASSUNTO(S)

tecidos tissues voltametria body fluids fluidos e humores no organismo antioxidants voltammetry exercise exercicios fisicos antioxidantes

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