Estudo comparativo de fatores de virulência em isolados clínicos de Pseudomonas aeruginosa produtores e não produtores de metalo- ß-lactamase SPM-1

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

29/05/2009

RESUMO

The invasion abilities, colonization and tissue damage presented by P. aeruginosa are due to the multiples virulence factors produced by the microorganism during the pathogenesis. Clinical isolates of SPM-1 metallo-β-lactamase Pseudomonas aeruginosa (SPM-1 MβL) from nosocomial infections present high rates morbidity and mortality and represent epidemic risk. The objective of this study was to research the expression of the virulence factors: alkaline protease, phospholipases C, elastases, pyocyanin, pyoverdin, hemolysins, biofilm formation in polystyrene microplate and the presence of the correlated genes aprA, lasA, lasB, plcN, plcH, toxA and algD. Ninety-eight isolated of P. aeruginosa obtained from several clinical specimens were studied, being 39 SPM-1 MßL producers, 20 imipenem intermediate or resistant SPM-1 MßL non-producers (IPM I/R) and 39 isolates imipenem susceptible SPM-1 MßL non-producers (IPM S). Correlating the phenotypic detection frequency of the different virulence factors studied among the groups SPM-1, IPM I/R and IPM S was observed statistical significance only for hemolysins production (P = 0.001). Comparing the detection of the analyzed virulence genes among the three groups of P. aeruginosa were not observed statistical relevance.The expression of most of the virulence factors was observed in varied percentages in all the isolates of the clinical materials. In agreement with the found results, it was verified that the virulence of the P. aeruginosa isolates not depends on the resistance presented to the carbapenems and that the virulence factors researched are important in all the analyzed clinical materials.

ASSUNTO(S)

pseudomonas aeruginosa microbiologia antibióticos beta-lactamicos microbiology beta-lactam antibiotics

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