Estudo biomonitorado para avaliação das atividades antiinflamatória e analgésica da folhas da espécie Protium spruceanum (Benth) Engler. (Burseraceae)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The aim of this work was to perform the bioactivity study of analgesic and antiinflammatory activities in vivo and antioxidant property in vitro of crude ethanolic extract (EEB) from Protium spruceanum leaves. Were used for phytochemical screening tests to detect the main classes of secondary metabolites presents in the EEB and chromatographic techniques for isolation of the major constituents of the most active fractions. The content of FVT and FT were determined by spectroscopic methods. For evaluation of pharmacological activities in vivo was utilized paw edema induced by carrageenan method to determine the anti-inflammatory activity and methods of writhing induced by acetic acid, hot plate and formalin for determination of antinociceptive activity. The acute toxicity was determined to assess the toxic effects of EEB. The phytochemical screening revealed the presence of phenolic compounds, tannins, flavonoids, saponins, triterpenes, steroids, coumarins and xanthones. The major constituent found in FHEX fraction was a mixture of α and β-amyrin. In the fractions FDCM and FMEOH-F were isolated three flavonoids that are still being identified. Bether antioxidant properties were observed for EEB and the more polar fractions FMEOH-1, FDCM and PDCM compared to the standard gallic acid. FDCM showed the highest activity. The EEB showed approximately 18% of phenolics compounds and in FDCM was observed the highest level of FVT. FHEX and FMEOH-1 showed antiiflammatory activity and after fractionation, FMEOH-F and FDCM showed significant antiinflammatory activity. In the writhing test, both fractions, FHEX and FMEOH-1 presents significative activity. For the hot plate method the EEB, in the highest dose administered, has been able to increase the TL initial 60 minutes after the administration and after fractionation, only FHEX showed significative activity. For the formalin method, the EEB and FHEX showed antinociceptive activity only in Phase II. The central and peripheral analgesic and anti-inflammatory of FHEX can be attributed to the presence of α and β- amyrin, the main constituent of this fraction. The anti-inflammatory activity of FMEOH-1, FMEOH-F and FDCM fractions can be attributed to the considerable amounts of phenolic compounds, especially flavonoids, in these fractions. It was impossible to establish the DL50 in mice for EEB until the administration of the dose of 6 g / kg.

ASSUNTO(S)

analgésicos plantas medicinais antiinflamatórios farmacologia

ACESSO AO ARTIGO

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