Estereologia, imuno-histoquímica e microscopia eletrônica de varredura na análise da reação tecidual a implantes de discos de adesivo dentinário fotoativado, hidróxido de cálcio e cimento de ionômero de vidro: estudo experimental em ratos / Stereology, imunohistochemistry, and scanning electron microscopy in the analysis of tissue reaction to disks implants of light-cured dentin adhesive, calcium hydroxide, and glass ionomer cement: experimental study in rats

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Purpose: to evaluate the biocompatibility of some pulpar protecting materials in the subcutaneous tissue of rats along the time. Methods: standardized implants of Calcium Hydroxide (CH), Glass Ionomer Cement (GIC) and Light-cured Dentin Adhesive (LDA) surgically were put in the back of Wistar rats and left by 15 and 30 days (each group with 5 animals) and the respective sham groups (S). After the experimentation the animals were sacrificed and material from the back of the animals was removed for study with light microscope, stereology and immunohistochemistry for smooth muscle alfa-actin (SMAA, myofibroblast detection). The implants and the fibrous capsule surfaces were analyzed with the scanning electronic microscopy (SEM) adapted with microanalysis. Results: the fibrosis and the number of mastocytes were different in the implant area depending of the implant type. Myofibroblasts were not detected expressing SMAA. The smallest value of the volume density of interstitial fibrosis (Vv[f]) occured in the CH group, and the largest in the LDA group (the difference was significant in relation to the S group, in the two experimental times, p <0.05). However, to the 30 days, the Vv[f] of the LDA group increase 30%, being different from the CH group in this period. The numerical density of mastocytes per area (NA[mastocyte]) was smaller in the CH group without difference for the S group, but with difference from the LDA group (this group had the largest NA[mastocyte] value in the two experimental times). The GIC group had, for Vv[f] and NA[mastocyte] all experimentation time, intermediary values ranging from CH to LDA values. There was discreet and restricted fibrosis in the surgical area with few mastocytes associated to vessels in the S group. There was positive correlation for Vv[f] and NA[mastocyte] in the LDA group. In the CH group the SEM observed interaction between the implant material and its fibrotic capsule, but not in the LDA and GIC groups. Conclusion: The studied materials induced the fibrosis formation and mastocytes migration in a differentiated way. The results suggest that CH as the most biologically compatible material among the materials tested in this work.

ASSUNTO(S)

cimento de ionômero de vidro imunohistoquímica light-cured dentin adhesive hidróxido de cálcio microscopia eletrônica de varredura glass ionomer cement biocompatibility adesivo dentinário fotoativado biocompatibilidade odontologia stereology estereologia materiais dentários - biocompatibilidade calcium hydroxide

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