Estabelecimento e caracterização de células-tronco fetais de membrana amniótica de cão / Establishment and characterization of fetal stem cells from amniotic dog\ s membrane

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

15/03/2012

RESUMO

The human amniotic membrane has taken an extremely important role in regenerative medicine in recent years, especially in dermatology and ophthalmology, and its promising use in treating diseases for which current therapy is ineffective. In addition, the amniotic membrane has human mesenchymal stem cells, which exhibit plasticity and have been applied to tissue regeneration. However, very little is known about the plastic capacity and the use of stem cells from amniotic membrane of animals, since the literature in this regard, it is not as broad as those of humans. In this project we established a culture of amniotic stem cells of dog, characterizing these cells in vitro and in vivo. The cells were obtained from a surgical procedure for hysterectomy in pregnant bitches, during castration\ s campaigns of the São Paulo\ s municipality. The cells were characterized in vitro, observing characteristics similar to other mesenchymal stem cells. In spite of their behavior, in vivo we observed the formation of a fast-growing tumor about a month after the inoculation of these cells in 10 immunosuppressed mice, being the tumor identified histologically as an embryonic carcinoma. Considering the biological behavior of forming a tumor in vivo, we infer that stem cells from amniotic membrane of dogs should not be used for application for therapeutic purposes in animals, at least until other collections are carried out so that it can be confirmed or not.

ASSUNTO(S)

amniotic membrane cão carcinoma carcinoma embrionário cellular therapy células-tronco dog membrana amniótica terapia celular

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