Espectroscopia de fluorescência na detecção de lesões quimicamente induzidas por agente carcinogênico na borda lateral da língua-estudo in vivo / Fluorescence spectroscopy to detection of chemically induced lesion at the lateral vorder of the tongue - in vivo study.

AUTOR(ES)

Cristina Kurachi

DATA DE PUBLICAÇÃO

2005

RESUMO

The diagnostic validity of the fluorescence spectroscopy in the discrimination of induced lesions at lateral border of the tongue of hamsters was investigated in this study. The animal model using chemical induction with DMBA (dimethylbenzanthracene) was chosen for the carcinogenesis phases comparable to the ones observed at oral cancer development in humans. The experimental groups were evaluated at each two weeks after the first four induction weeks until a total of 26 weeks. The fluorescence spectroscopy system employed is composed by: excitation laser, Y-type investigation probe, spectrometer and a computer. Two excitation wavelengths were used: 442 and 532 nm. The investigation probe has a central fiber for laser delivering and six concentric ones for reemitted light collection. The fluorescence is analyzed in the spectrometer in the 378-842 nm range. The optical investigation is punctual and the result is one spectrum per point. Then the animals were killed and the tongue removed for histopathological analysis. The spectra study was performed during the entire carcinogenesis process and the final analysis comparing to clinical and histopathological results. The spectra were evaluated in two ways: intra-spectral correlation analysis and inter-spectral analysis. The 442 nm excitation induced the best normal/carcinoma discrimination. The best result was achieved with the KNN analysis with 95.7% of sensitivity, 91.6% of specificity and 94% of accuracy. The discrimination using the intra-spectral analysis obtained 90.9%, 81.5% and 87.1% of sensitivity, respectively. However, using a discerning determination of the discrimination limit for classification it was possible to achieve similar results between the two employed analyses.

ASSUNTO(S)

detection cancer diagnosis língua. tongue. diagnóstico detecção fluorescence fluorescência câncer

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