ESCHERICHIA COLI K-12 Auxotrophs Induced by Insertion of the Transposable Element Tn5

AUTOR(ES)

Shaw, Karen J.

RESUMO

The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.

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