Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection.
AUTOR(ES)
Beards, G M
RESUMO
We describe two enzyme-linked immunosorbent assays for rotavirus antigen in feces, which were designed to be as sensitive and specific as possible, and easy to use anywhere. Both are indirect methods, using the antibody capture method, but the second assay utilizes a rotavirus group-specific monoclonal "detecting" antibody instead of the hyperimmune polyvalent guinea pig antisera used in the first assay. Both tests were found to be more sensitive than electron microscopy for detecting virus. To develop these tests, solid phase, antiserum production methods, treatment of the test antigen with EDTA, substrate, stability of reagents, and the need for confirmatory "blocking" tests were all examined. The first assay described is that used at present by the World Health Organization for their worldwide diarrheal disease control program.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=271031Documentos Relacionados
- Detection of staphylococcal enterotoxins by enzyme-linked immunosorbent assays and radioimmunoassays: comparison of monoclonal and polyclonal antibody systems.
- Detection of group C rotavirus antigens and antibodies in animals and humans by enzyme-linked immunosorbent assays.
- Enzyme-linked immunosorbent assays adapted for serotyping of human rotavirus strains.
- Monoclonal antibody-based enzyme-linked immunosorbent assays for identification and serotyping of Vibrio cholerae O1.
- Enzyme-Linked Immunosorbent Assays for Cholera Serology