Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
AUTOR(ES)
Yolken, R H
RESUMO
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=274794Documentos Relacionados
- Improved GM1-enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
- Rapid GM1-enzyme-linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin.
- Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin.
- GM1 ganglioside enzyme-linked immunosorbent assay for detection of heat-labile enterotoxin produced by human and porcine Escherichia coli strains.
- Evaluation of a ganglioside immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
