Enzymatic activity of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase produced by Gordonia polyisoprenivorans

AUTOR(ES)

Silva, Andréa Scaramal, Camargo, Flávio Anastácio de Oliveira, Andreazza, Robson, Jacques, Rodrigo Josemar Seminoti, Baldoni, Daiana Bortoluzzi, Bento, Fátima M.

FONTE

Quím. Nova

DATA DE PUBLICAÇÃO

2012

RESUMO

This study aimed to evaluate the environmental conditions for enzyme activity of catechol 1,2-dioxygenase (C1,2O) and catechol 2,3-dioxygenase (C2,3O) produced by Gordonia polyisoprenivorans in cell-free and immobilized extracts. The optimum conditions of pH, temperature, time course and effect of ions for enzyme activity were determined. Peak activity of C1,2O occurred at pH 8.0. The isolate exhibited the highest activity of C2,3O at pH 7.0 and 8.0 for the cell-free extract and immobilized extract, respectively. This isolate exhibited important characteristics such as broad range of pH, temperature and time course for enzyme activity.

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