Engineering high-level aluminum tolerance in barley with the ALMT1 gene
AUTOR(ES)
Delhaize, Emmanuel
FONTE
National Academy of Sciences
RESUMO
Acidity is a serious limitation to plant production on many of the world's agricultural soils. Toxic aluminium (Al) cations solubilized by the acidity rapidly inhibit root growth and limit subsequent uptake of water and nutrients. Recent work has shown that the ALMT1 gene of wheat (Triticum aestivum) encodes a malate transporter that is associated with malate efflux and Al tolerance. We generated transgenic barley (Hordeum vulgare) plants expressing ALMT1 and assessed their ability to exude malate and withstand Al stress. ALMT1 expression in barley conferred an Al-activated efflux of malate with properties similar to those of Al-tolerant wheat. The transgenic barley showed a high level of Al tolerance when grown in both hydroponic culture and on acid soils. These findings provide additional evidence that ALMT1 is a major Al-tolerance gene and demonstrate its ability to confer effective tolerance to acid soils through a transgenic approach in an important crop species.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=524075Documentos Relacionados
- High-Level Acetaldehyde Production in Lactococcus lactis by Metabolic Engineering
- High-level ribosomal frameshifting directs the synthesis of IS150 gene products.
- High-Level Production of Recombinant Human Parathyroid Hormone 1-34
- High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.
- Detection of the High-Level Aminoglycoside Resistance Gene aph(2")-Ib in Enterococcus faecium