Engineering Desiccation Tolerance in Escherichia coli
AUTOR(ES)
Billi, Daniela
FONTE
American Society for Microbiology
RESUMO
Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (P 000000000000 000000000000 000000000000 111111111111 000000000000 111111111111 000000000000 000000000000 000000000000 O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.
