Endo-and exoglucanase activities in bacteria from mangrove sediment
AUTOR(ES)
Soares Júnior, Fábio Lino, Dias, Armando Cavalcante Franco, Fasanella, Cristiane Cipola, Taketani, Rodrigo Gouvêa, Lima, André Oliveira de Souza, Melo, Itamar Soares, Andreote, Fernando Dini
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2013-09
RESUMO
The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose.
Documentos Relacionados
- Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities.
- Hydrocarbon Mineralization in Sediments and Plasmid Incidence in Sediment Bacteria from the Campeche Bank
- Evaluation of petroleum-degrading potential of bacteria from water and sediment.
- Endo-1,4-[beta]-Glucanase, Xyloglucanase, and Xyloglucan Endo-Transglycosylase Activities Versus Potential Substrates in Ripening Tomatoes.
- Evidence for Detachment of Indigenous Bacteria from Aquifer Sediment in Response to Arrival of Injected Bacteria