End Products of Glucose Fermentation by Brochothrix thermosphacta
AUTOR(ES)
Grau, Frederick H.
RESUMO
Anaerobically, Brochothrix thermosphacta fermented glucose primarily to l-lactate, acetate, formate, and ethanol. The ratio of these end products varied with growth conditions. Both the presence of acetate and formate and a pH below about 6 increased l-lactate production from glucose. Small amounts of butane-2,3-diol were also produced when the pH of the culture was low (≤5.5) or when acetate was added to the growth medium. Radioactive label from [1-14C]acetate was incorporated into ethanol and l-lactate, implying reversibility of pyruvate-formate lyase. In crude extracts, the following enzymes involved in pyruvate metabolism were demonstrated: lactate dehydrogenase, phosphotransacetylase, acetate kinase, acetaldehyde dehydrogenase (coenzyme A acetylating), ethanol dehydrogenase, pH 6 acetolactate-forming enzyme, and diacetyl (acetoin) reductase. The lactate dehydrogenase did not require fructose-1,6-disphosphate or Mn2+ for activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=242234Documentos Relacionados
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