Emprego das aberraÃÃes cromossÃmicas instÃveis e micronÃcleos no biomonitoramento individual: estudo comparativo

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Biodosimetry is based on the investigation of radioinduced biological effects in order to correlate them with the absorbed dose. The quantification of unstable chromosome aberrations and micronuclei, in peripheral blood lymphocytes, are two methods commonly used in biodosimetry. In this context, the aim of this research was to compare these methods in the biomonitoring of health care professionals occupationally exposed to ionizing radiation. In parallel, the technique of C-banding was evaluated for quality control of unstable chromosome aberrations analyses. Thus, samples of peripheral blood from health care professionals of three hospitals from Recife (Brazil) were collected, and the lymphocytes cultures were carried out based on the cytogenetic classical technique. It was pointed out that analysis of micronuclei is faster than the unstable chromosome aberrations ones, which suggests the use of the former in preliminary evaluation in cases of suspected accidental exposure. C-banding technique was efficient, as confirmatory test, in the identification of dicentrics and rings during the analyses of unstable chromosome aberrations, being able to be applied in the quality control in biodosimetry. The comparison between the individual work conditions with the frequencies of unstable aberrations and micronuclei, obtained from cytogenetic analysis, resulted in the change of behavior of the professionals involved in this research, with a better observance of the radioprotection standards.

ASSUNTO(S)

biomonitoramento engenharia nuclear cromossomo aberraÃÃes

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