Embryonic development of the egg of Schistosoma mansoni Sambon, 1907. / Desenvolvimento embrionário do ovo de Schistosoma mansoni Sambon, 1907.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

In pathogenesis of schistosomiasis and in its life cycle, the eggs have a central role. Traditionally, the primary screening of antischistosomal drugs uses as main criteria the Vogel and Pratas classification (VPs), which is based on the relation of embryo/eggshell sizes and the qualitative oogram. However, little is known on egg developmental biology itself so far. The present work aimed to describe the morphological events during the Schistosoma mansoni egg embryogenesis by using conventional histology and whole-mount preparations. These events were divided in ten stages, adapting a previous classification initially introduced for a free-living rhabdocoel. Egg maturation in the host and in culture (RPMI-1640 medium) were also evaluated by statistical modeling of the mean area for each stage (considering VPs classification) and then morphologicaly by whole-mount eggs under the confocal laser microscopy (our proposed staging system herein). Due to difficult recognition of the morphological events in live, unstained eggs, a further correlation to the VPs classification is presented. Indeed, schistosome egg embryonic development is more complex than previous considered (as in VPs classification). The differentiation of embryonic structures and their probable role in granulomatogenesis is discussed. The stage system here proposed may contribute to a better comprehension of the egg biology and underlies further studies on egg antigen production and its diffusion in the the granuloma reaction.

ASSUNTO(S)

desenvolvimento embrionário granuloma granuloma schistosoma mansoni embryonic development parasitologia animais schistosoma mansoni

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