Electrical Impedance Measurements in the Reading and Monitoring of Broth Dilution Susceptibility Tests

AUTOR(ES)

Colvin, H. J.

RESUMO

Electrical impedance changes in the medium were studied during traditional broth dilution tests. Tests involved clinical isolates of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter sp., Klebsiella, and enterococcus and ten antibiotics. Minimal inhibitory concentration (MIC) values from overnight visual readings were compared with MIC values determined from electrical impedance end points, using inocula of 106 organisms per ml. Ninety-three percent of the results were within one twofold dilution of each other. When the impedance end point was determined at 6 h, this correlation was lowered to 34%. By increasing the initial electrical impedance inoculum 10-fold, the correlation between the 6-h impedance MIC and the overnight visual MIC was improved to 74%. Ampicillin, tetracycline, and polymyxin E results accounted for most discrepancies. Continuous monitoring of impedance changes suggested that better correspondence could be obtained by adjusting the end point criteria for the 6-h impedance MIC with different antibiotics. Electrical impedance methods for reading bacterial end points in automated clinical laboratory instruments appear promising.

Documentos Relacionados

• Electrical impedance measurements: rapid method for detecting and monitoring microorganisms.
• Comparison of the agar dilution, tube dilution, and broth microdilution susceptibility tests for determination of teicoplanin MICs.
• Comparison of agar dilution, tube dilution, and broth microdilution susceptibility tests for determination of ramoplanin MICs.
• Effect of inoculum size on results of macrotube broth dilution susceptibility tests with Branhamella catarrhalis.
• Results of a Survey of Antifungal Susceptibility Tests in the United States and Interlaboratory Comparison of Broth Dilution Testing of Flucytosine and Amphotericin B