Eficacia de limpadores quimicos a base de peroxidos e hipoclorito na remoção de Candida spp. em rembasadores resilientes / Efficacy of denture cleansers on denture liners contaminated with Candida species

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Candida albicans is associated with denture stomatitis etiology, pathology which affect about 11 to 67% of removable prostheses users. However, more recently, the Candida glabrata has been highlighted for presenting superior hydrophobicity and resin acrylic surface adherence when compared to the C. albicans. In addition, surface characteristics materiais such roughness (Ra) and surface free energy (EL8) may contribute to the microorganisms adhesion. Thus, the purpose of this study was to evaluate the surface roughness and surface free energy of methyl methacylate liners materiais (Coe 80ft and Kooliner) and silicone (Ufi Gel P) before contamination with C. albicans (ATCC 90028) and C. glabrata (ATCC2001), as well to verify the peroxide chemical denture cleansers efficacy (Polident 3 minutos and Efferdent) and 0.5% sodium hypochlorite - NaOCl, in the miaoorganisms removing. Thus, 64 rectangular bases measuring 25 x 12 x 1 mm using microwave-polymerized acryli~ resins, following manufacturers recommendations, to each material were made, then were relined and after surface roughness and surface free energy were measured. Next, the samples were randomly separated by lottery into two groups of 32 each, according to the fungus and these were subdivided into four groups of eight as the treatments: G1 - Distilled water (Control); G2 - Polident 3 min. utes; G3 - Efferdent; G4 - 0,5% NaOCI. Ali the samples rested in human whole saliva for 30 minutes to form an acquired pellicle. After, they were submitted to the adherence assay with one of the fungus for two hours, and then, treated, following these times: G1 - Distilled water (15 minutes); 2 - Polident 3 minutes (3 minutes); 3 - Efferdent (15 minutes); 4 - 0,5% sodium hypochlorite (10 minutes). The adhered cells were counted using a light microscope (Axiostar 2 Plus, Carl Zeiss, Jena, Germany) at 400 x magnification. The data were submitted to the analysis of variance and Tukey test (roughness and fungus adherence) and ANOVA on Ranks for EL8, with significance levei of 5%. The silicone-based liner (Ufi Gel P) showed lower values of roughness compared to the methyl methacrilate-based liners (Coe 80ft and Kooliner), (p<0.05). However, ali these materiais were different among them for surface free energy (p<0.05), where the Coe 80ft and Ufi Gel P showed the highest and lowest values, respectively. Candida glabrata showed the highest number of adhered cells for ali materiais (p<0.01). Among evaluated cleansers, only 0.5% sodium hypochlorite differed from the control (p=0.001), showing the lowest number of remaining cells adhered. The conclusion is that the 0.5% sodium hypochlorite was the only one chemical cleanser efficient in the adhered cells removing in ali denture liners independent of the Candida specie.

ASSUNTO(S)

candida albicans candida albicans candida glabrata candida glabrata

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