Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR

Leão, Evelynne Urzêdo, Spadotti, David Marques de Almeida, Rocha, Kelly Cristina G., Lima, Élison Fabricio B., Tavella, Luciana, Turina, Massimo, Krause-Sakate, Renate

Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR

AUTOR(ES)

Leão, Evelynne Urzêdo, Spadotti, David Marques de Almeida, Rocha, Kelly Cristina G., Lima, Élison Fabricio B., Tavella, Luciana, Turina, Massimo, Krause-Sakate, Renate

FONTE

Braz. arch. biol. technol.

DATA DE PUBLICAÇÃO

14/06/2018

RESUMO

ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.