Effects of supplementation with L-Glutamine and L-alanyl-Lglutamine upon parameters of muscle damage and inflammation in trained rats and submitted to intense exercise of swim / Efeito da suplementação com L-glutamina e L-alanil-L-glutamina sobre parâmetros de lesão muscular e de inflamação em ratos treinados e submetidos a exercício intenso de natação

AUTOR(ES)

Vinicius Fernandes Cruzat

DATA DE PUBLICAÇÃO

2008

RESUMO

Glutamine is the most abundant free amino acid found in plasma and muscle, and is utilized at high rates by many cells for maintenance and promotion of cell function. However, in some catabolic situations, like intense and prolonged or exhaustive physical exercise, a reduction in GLN availability is observed, fact that may impair the cell functions. Oral supplementation with dipeptides of glutamine, like L-alanyl-L-glutamine may serve as an interesting way to deliver glutamine to the organism. Male Wistar rats were trained and supplemented with DIP (1,49 g•kg-1) or mixture containing free L-glutamine (1 g•kg-1) and Lalanine (0,61 g•kg-1) or water (CON) and sacrificed in distinct times: before (TR) and immediately after (INT) intense exercise of swim. Plasma and serum concentrations of glucose, GLN, glutamate, ammonia, CK, LDH, TNF-• and PgE2 were evaluated. In muscles (soleus and gastrocnemius) and liver GLN, glutamate, GSH, GSSG and total proteins also were evaluated. Low concentration of CK in plasma was observed in both supplemented groups before the exhaustive test. The DIP-TR presented more concentration of glutamate, GSH e GSH/GSSG in the soleus muscle and liver. Higher concentration of glutamine, glutamate and GSH/GSSG was seen in GLN+ALATR group. Both groups supplemented with DIP and GLN+ALA after the exhaustive test, exhibit decreases in ammonia and TNF-α, and the group DIP-INT, demonstrated decrease in PgE2. Glutamine, glutamate and GSH/GSSG increases in the soleus and glutamate in the gastrocnemius increases in groups DIP-INT and GLN+ALA-INT. Increases in GSH and GSH/GSSG in liver were found in the same groups, compared to CON-INT. Our results indicate that oral supplementation with free L-glutamine added to L-alanine or the dipeptide, Lalanyl-L-glutamine, may influence the muscle and liver stores of GSH and the cellular redox state, and this may attenuate damage and inflammation induced by severe physical exercise.

ASSUNTO(S)

dipeptide glutamine dipeptídeo nutrição experimental glutamina inflammation muscle damage lesão muscular suplementação alimentar inflamação gsh gsh

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