Effects of laboratory maintenance on the nature of surface reactive antigens of Neisseria gonorrhoeae.
AUTOR(ES)
Arko, R J
RESUMO
The extensive in vitro cultivation methods used in propagating and maintaining gonococcal cells were found to affect their virulence, antigenicity, and ultrastructure. Adapting a laboratory-maintained strain of gonococci to animal virulence resulted in two lines of pilated cells with similar colonial morphologies. The animal-adapted cells, however, had a greater amount of extracellular pili and a more prominent peptidoglycan cell wall layer. They were also more resistant to the bactericidal effects of guinea-pig complement and more reactive in macroagglutination and bactericidal tests with strain-specific gonococcal antibody. In comparative guinea-pig protection trials, formalin-fixed cells of the animal-adapted cell line were 500 times more effective as immunogens than the laboratory-maintained cell line.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1045292Documentos Relacionados
- Effects of steroid hormones on Neisseria gonorrhoeae.
- Laboratory detection of ciprofloxacin resistant Neisseria gonorrhoeae.
- Effect of iron on surface charge and hydrophobicity of Neisseria gonorrhoeae.
- Effects of amino acids on colony phenotype of Neisseria gonorrhoeae.
- Wheat-germ agglutination of Neisseria gonorrhoeae. A laboratory investigation.