Effects of histone acetylation on chromatin topology in vivo.
AUTOR(ES)
Lutter, L C
RESUMO
Recently a model for eukaryotic transcriptional activation has been proposed in which histone hyperacetylation causes release of nucleosomal supercoils, and this unconstrained tension in turn stimulates transcription (V. G. Norton, B. S. Imai, P. Yau, and E. M. Bradbury, Cell 57:449-457, 1989; V. G. Norton, K. W. Marvin, P. Yau, and E. M. Bradbury, J. Biol. Chem. 265:19848-19852, 1990). These studies analyzed the effect of histone hyperacetylation on the change in topological linking number which occurs during nucleosome assembly in vitro. We have tested this model by determining the effect of histone hyperacetylation on the linking number change which occurs during assembly in vivo. We find that butyrate treatment of cells infected with simian virus 40 results in hyperacetylation of the histones of the extracted viral minichromosome as expected. However, the change in constrained supercoils of the minichromosome DNA is minimal, a result which is inconsistent with the proposed model. These results indicate that the proposed mechanism of transcriptional activation is unlikely to take place in the cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360433Documentos Relacionados
- Effect of transcription of yeast chromatin on DNA topology in vivo.
- Effect of histone acetylation on structure and in vitro transcription of chromatin.
- Targeted chromatin binding and histone acetylation in vivo by thyroid hormone receptor during amphibian development
- Histone acetylation: a switch between repressive and permissive chromatin: Second in review series on chromatin dynamics
- Histone H1 Is a Specific Repressor of Core Histone Acetylation in Chromatin
