Effects of alveolar bone displacement with segmental osteotomy: micro-CT and histomorphometric analysis in rats
KIM, Taegun, LEE, Won, BAEK, Sang-Ho, PYO, Sungwoon, KOOK, Yoon-Ah, BAYOME, Mohamed, KIM, Insoo
Braz. oral res.
DATA DE PUBLICAÇÃO
Abstract The purpose of this study was to evaluate the effects of segmental osteotomy on the blood vessels and osteoclasts in rats using micro-computed tomography (micro-CT) and histomorphometric analysis. After segmental osteotomy was performed around the maxillary first molars of 36 male Sprague-Dawley rats (n = 72), the samples were divided into a control group (no displacement), 0.5 D group (0.5 mm buccal displacement) and 1.0 D group (1.0 mm buccal displacement) (n = 24/group). At 1, 2, 4 and 8 weeks after surgery, changes in the blood vessel volume were investigated using micro-CT with perfusion of radiopaque silicone rubber. Tartrate-resistant acid phosphatase (TRAP) staining was used for histomorphometric analysis. Two-way repeated measures analysis of variance (rmANOVA) was performed to compare the volume of blood vessels and number of TRAP-positive osteoclasts among the groups. Regarding blood vessel volume, the displacement groups had no significant effects, while the time points had significant effects (p = 0.014). The blood vessel volume at 1 week was significantly smaller than that at 2, 4, and 8 weeks (p = 0.004, p = 0.026, and p = 0.005, respectively). Regarding TRAP cell count, the displacement groups had no significant effects, while the time points had significant effects (p < 0.001). The number of TRAP-positive osteoclasts at 8 weeks was significantly smaller than that at 1, 2, and 4 weeks (p < 0.001, p < 0.001, and p = 0.002, respectively), and the count at 4 weeks was smaller than that at 1 week (p = 0.011). Therefore, a regional osteoclast-related acceleratory phenomenon was maintained until 4 weeks after surgery.
- Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis
- Apical Displacement and Residual Root Canal Filling with Single-Cone After Post Space Preparation: A Micro-CT Analysis
- Three-dimensional measurement of alveolar airspace volumes in normal and emphysematous lungs using micro-CT
- Impact of micronutrients supplementation on bone repair around implants: microCT and counter-torque analysis in rats
- Micro-CT assessment of two different endodontic preparation systems