Effects of a commercial biocide, kasugamycin and consistency of the culture medium on the in vitro establishment of bamboo1
AUTOR(ES)
Santos, Daniela Werner Ribeiro dos
FONTE
Pesqui. Agropecu. Trop.
DATA DE PUBLICAÇÃO
25/11/2019
RESUMO
RESUMO A contaminação por micro-organismos e oxidação dos explantes no estabelecimento in vitro de bambu são problemas recorrentes na sua micropropagação. Avaliaram-se os efeitos do biocida Plant Preservative Mixture (PPM®), do antibiótico casugamicina e da consistência do meio de cultura no estabelecimento in vitro de Bambusa vulgaris,Phyllostachys bambusoides e Dendrocalamus asper. A presença de PPM® no meio de cultura apresentou efeito significativo nas concentrações de 2 mL L-1 ou 4 mL L-1, assim como o meio de cultura líquido no aumento de plantas estabelecidas no outono. A casugamicina promoveu respostas variáveis para as três espécies, dependendo da estação do ano. Houve interação entre os fatores, demonstrando que maiores índices de plantas viáveis podem ser obtidos aliando-se diferentes estratégias para diminuir a oxidação e a contaminação. Para o estabelecimento in vitro de B. vulgaris,P. bambusoides e D. asper, é recomendada a adição de 2 mL L-1 ou 4 mL L-1 de PPM® ao meio de cultura líquido.ABSTRACT The contamination by microorganisms and oxidation of explants in the in vitro establishment of bamboo are recurrent problems for its micropropagation. In the present study, effects of the biocide Plant Preservative Mixture (PPM™), the antibiotic kasugamycin and the consistency of the culture medium were evaluated in the in vitro establishment of Bambusa vulgaris,Phyllostachys bambusoides and Dendrocalamus asper. The presence of PPM™ in the culture medium had a significant effect using 2 mL L-1 or 4 mL L-1 concentrations, as well as in the liquid culture medium, increasing the plants established in the autumn. Kasugamycin promoted variable responses for all the three species, depending on the season. There was interaction among the factors, demonstrating that higher rates of viable plants can be obtained by combining different strategies to reduce the oxidation and contamination. For the in vitro establishment of B. vulgaris,P. bambusoides and D. asper, it is recommended to add 2 mL L-1 or 4 mL L-1 of PPM™ to the liquid culture medium.
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