Effect of the hydroethanolic extract of Baccharis trimera on pregnant rats and conceptus. / Efeito do extrato hidroetanólico de Baccharis trimera em ratas prenhes e seus conceptos

AUTOR(ES)

Simone Reschke Mendes Grance

DATA DE PUBLICAÇÃO

2007

RESUMO

The aim of this investigation was to evaluate the effect of the hydroethanolic extract of Baccharis trimera on pregnant Wistar rats. The 35 female rats evaluated and distributed into three groups. Those in treatment groups 1 and 2 were given 8.4 mg/kg of the extract orally from gestational day (GD) 1 to 19 and from GD 6 to 15, respectively, whereas those in control group received distilled water orally from GD 1 to 19. Body weights were recorded on GD 1, 6, 15, and 20. On GD 20 all animals were anesthetized, blood samples were collected and maternal liver, kidneys and spleen were weighed and processed for histological studies. No clinical signs of maternal toxicity were observed. No changes in corrected body weight or in hematological parameters were found. Urea levels and kidneys weight differed significantly between treatment 1 animals and controls. No histopathological alterations were found in kidneys or liver in both treatment groups. The results revealed that the hydroethanolic extract of B. trimera administered to pregnant rats at a concentration of 8.4 mg/kg is toxic to maternal kidney and liver cells, although the alterations are reversible once administration is discontinued. The present study investigated the hydroethanolic extract of Baccharis trimera on pregnant rats when administered at a concentration of 8.4 mg/kg from gestational day (GD) 1 to 19 and 6 to 15 (treatments 1 and 2, respectively), as compared to controls given distilled water. Maternal body weights were recorded on GD 1, 6, 15 and 20. Ovaries and uterus were weighed; liver and kidneys were weighed and examined microscopically; fetuses were counted, weighed, measured, and evaluated for external, visceral, and skeletal alterations. Changes were found in kidneys weight in treatment 1 and in hepatorenal histopathological features in both treatments. Implantation rate was decreased and preimplantation loss rate was increased in treatment 1. Placentomegaly was detected in both treatments, whereas in treatment 1 visceral malformations and skeletal variations were increased and fetal weight and size and ossification were decreased. The results revealed that the hydroethanolic extract of B. trimera at the concentration tested exhibits maternal and embryofetal toxicity, although the alterations observed in the offspring may not be a direct effect of the extract, but rather a consequence of its toxicity against the maternal organism.

ASSUNTO(S)

embriotoxicidade fetotoxicidade extrato hidroetanólico embryotoxicity carqueja - toxicologia fetotoxicity asteraceae rattus norvegicus hydroethanolic extract toxicologia animal rato - feto rato como animal de laboratório

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