Effect of peracetic acid on biofilms formed by Listeria monocytogenes strains isolated from a Brazilian cheese processing plant
Lee, Sarah Hwa In, Barancelli, Giovana Verginia, Corassin, Carlos Humberto, Rosim, Roice Eliana, Coppa, Carolina Fernanda Sengling Cebin, Oliveira, Carlos Augusto Fernandes de
Braz. J. Pharm. Sci.
DATA DE PUBLICAÇÃO
ABSTRACT This study aimed to investigate the effect of peracetic acid (PAA, 0.5%) on adherent cells of three strains of Listeria monocytogenes strains belonging to serotypes 4b and 1/2b that had been previously isolated from the environment of a Brazilian cheese plant. The assays were conducted using polystyrene microplates and stainless steel coupons and the adhered cells were treated with PAA for 60, 120 and 180 s. On stainless steel, biofilms were partially inactivated by PAA after 60 s and almost 100% of the cells were damaged within 180 s using epifluorescence microscopy with LIVE/DEAD® staining. On polystyrene microplates, PAA decreased (P<0.05) biofilm biomass produced by the three L. monocytogenes isolates at 60 s, when compared with controls (no PAA treatment). However, PAA did not completely eliminate L. monocytogenes cells on polystyrene microplates (decreasing 1.8-2.5 log cycles after treatment with PAA for 180 s). The correct concentration and contact time of PAA is critical for eliminating biofilms formed by L. monocytogenes on stainless steel surfaces, although further studies are needed for defining efficient PAA treatments to remove adherent cells of this pathogen on plastic polymers.
- Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces
- Effect of Food Processing-Related Stresses on Acid Tolerance of Listeria monocytogenes
- Methods for improved recovery of Listeria monocytogenes from cheese.
- Effect of Antimicrobials on Salmonella Spp. Strains Isolated from Poultry Processing Plants
- Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from 1958 to 1982 in Sweden.