Effect of Mycobacterium tuberculosis-derived sulfolipid I on human phagocytic cells.

AUTOR(ES)

Zhang, L

RESUMO

Experiments were performed to determine the effects of Mycobacterium tuberculosis-derived sulfolipid I on phagocytic cells. Sulfolipid I was taken up in significant amounts by human neutrophils and in lesser amounts by monocytes and lymphocytes. Superoxide (O2-) production by neutrophils was significantly increased by sulfolipid I, but the rate of production was slower than that reported previously for other stimuli. The optimal concentration of sulfolipid I for stimulation of O2- production was 27 micrograms/ml, while higher concentrations produced less. At substimulatory levels sulfolipid I caused enhancement of O2- release from neutrophils when it was subsequently stimulated by other agents. Nonadherent monocytes from most normal donors failed to produce O2- when treated with sulfolipid I; however, adherent monocytes pretreated with gamma interferon did produce O2- with sulfolipid I stimulation. Priming for an enhanced oxidative response of activated monocytes was also observed. These sulfolipid I-induced changes in phagocytic cell function may be important in altering the ability of phagocytes to respond effectively to M. tuberculosis and may also cause exaggerated inflammatory responses.

Documentos Relacionados

• Modulation of macrophage lysosomal pH by Mycobacterium tuberculosis-derived proteins.
• Identification of HLA class II-restricted determinants of Mycobacterium tuberculosis-derived proteins by using HLA-transgenic, class II-deficient mice
• Mycobacterium tuberculosis alters expression of adhesion molecules on monocytic cells.
• Stimulatory effect of inflammatory cytokines on alpha 1-antichymotrypsin expression in human lung-derived epithelial cells.
• STUDIES ON THE METABOLISM OF MYCOBACTERIUM TUBERCULOSIS I. : The Effect of Carbohydrates and Alcohols on the Growth of Mycobacterium tuberculosis var. hominis1