Effect of light on the attachment of cyanophage AS-1 to Anacystis nidulans.

AUTOR(ES)

Cséke, C S

RESUMO

The effect of illumination on the extent and kinetics of the adsorption of cyanophage AS-1 to the blue-green alga (cyanobacterium) Anacystis nidulans was studied by using 32P-labeled phage. The initial rate of adsorption was not significantly affected by light. However, at Na+ levels used ordinarily to culture the alga ([Na+] = 11.7 mM), the total amount of phage adsorbed was doubled in the illuminated cultures, as compared with the dark-grown ones, over a wide range of multiplicities of infection (0.05 to 20). Upon a 10-fold increase in Na+ concentration in the medium ([Na+] = 0.11 M), the dark adsorption of the phage increased to the level of light adsorption found in low Na+ medium. The effects on phage adsorption of high Na+ concentration and light were not additive.

Documentos Relacionados

• Adsorption of cyanophage AS-1 to unicellular cyanobacteria and isolation of receptor material from Anacystis nidulans.
• Ultraviolet Light Inactivation and Photoreactivation of AS-1 Cyanophage in Anacystis nidulans
• Effect of iron deficiency and iron restoration on ultrastructure of Anacystis nidulans.
• Labeling the deoxyribonucleic acid of Anacystis nidulans.
• Simple, effective method for purifying the AS-1 cyanophage.