Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury
AUTOR(ES)
Santos, Carlos Henrique Marques dos, Dourado, Doroty Mesquita, Silva, Baldomero Antonio Kato da, Pontes, Henrique Budib Dorsa, Azevedo Neto, Euler de, Vendas, Giovanna Serra da Cruz, Chaves, Ian de Oliveira, Miranda, João Victor Cunha, Oliva, João Victor Durães Gomes, Dias, Letícia do Espírito Santo, Almeida, Murillo Henrique Martins de, Sampaio, Trícia Luna
FONTE
Braz. J. Cardiovasc. Surg.
DATA DE PUBLICAÇÃO
2018-04
RESUMO
Abstract Objective: The aim of the present study was to evaluate the ability of ischemic postconditioning, atorvastatin and both associated to prevent or minimize reperfusion injury in the lung of rats subjected to ischemia and reperfusion by abdominal aortic clamping. Methods: We used 41 Wistar norvegic rats, which were distributed into 5 groups: ischemia and reperfusion (I/R), ischemic postcondictioning (IPC), postconditioning + atorvastatin (IPC+A), atorvastatin (A) and SHAM. It was performed a medium laparotomy, dissection and isolation of the infra-renal abdominal aorta; except for the SHAM group, all the others were submitted to the aortic clamping for 70 minutes (ischemia) and posterior clamp removal (reperfusion, 70 minutes). In the IPC and IPC+A groups, postconditioning was performed between the ischemia and reperfusion phases by four cycles of reperfusion and ischemia lasting 30 seconds each. In the IPC+A and A groups, preceding the surgical procedure, administration of 3.4 mg/day of atorvastatin was performed for seven days by gavage. After the surgical procedure, the right caudal lobe was removed from the lung for histological study, using tissue injury score ranging from grade 1 (normal tissue) to grade 4 (intense lesion). Results: The mean lung injury was 3.6 in the I/R group, 1.6 in the IPC group, 1.2 in the IPC+A group, 1.2 in the A group, and 1 in the SHAM group (P<0.01). Conclusion: Ischemic postconditioning and atorvastatin were able to minimize lung reperfusion injury, alone or in combination.
Documentos Relacionados
- Evaluation of the Effects of Atorvastatin and Ischemic Postconditioning Preventing on the Ischemia and Reperfusion Injury: Experimental Study in Rats
- Remote limb ischemic post-conditioning attenuates ischemia-reperfusion injury in rat skin flapby limiting oxidative stress
- Ischemic post-conditioning attenuates the intestinal injury induced by limb ischemia/reperfusion in rats
- Ischemic pre and postconditioning in skeletal muscle injury produced by ischemia and reperfusion in rats
- Neuroprotective effect of atorvastatin in spinal cord ischemia-reperfusion injury