Effect of Glucose and CO2 on Nitrate Uptake and Coupled OH− Flux in Ankistrodesmus braunii1

AUTOR(ES)

Eisele, Rolf

RESUMO

In Ankistrodesmus braunii, in the absence of CO2, i.e. in CO2-free air or N2, photosynthetic nitrate uptake and nitrate reduction were inhibited, especially at low pH. Under such conditions, glucose stimulated nitrate uptake and reduction to almost the same level in the pH range between 6 and 8.5. CO2 at 0.03% effected an intermediate pH dependence of nitrate uptake; saturating CO2 concentration (more than 1%) eliminated the pH dependence, as did glucose, but the rates were enhanced compared with glucose. Glucose and, even more, CO2, drastically reduced the release of nitrite and ammonia to the medium, the stoichiometry between alkalinization of the medium and nitrate uptake (OH−/NO3−) approached 1.

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