Effect of detergents on membrane-associated glucan synthetase from Paracoccidioides brasiliensis.

AUTOR(ES)

San-Blas, G

RESUMO

Yeast and mycelial particulate preparations of Paracoccidioides brasiliensis were subjected to the action of several detergents in an attempt to solubilize the glucan synthetase present in these preparations. This was achieved more successfully in the yeast membranes than in the mycelial ones. The enzymatic activity was greatly stimulated in the insoluble fractions upon treatment with some of the detergents used. The results suggest that the yeast and mycelial phases of P. brasiliensis may differ in the structures of their membranes and also in the characteristics of their glucan synthetases.

Documentos Relacionados

• Membrane-associated phosphatidylinositol kinase from Saccharomyces cerevisiae.
• Effect of membrane-associated f1 bacteriophage coat protein upon the activity of Escherichia coli phosphatidylserine synthetase.
• Effect of vinblastine on distribution of murine leukemia virus-derived membrane-associated antigens.
• Membrane-associated methane monooxygenase from Methylococcus capsulatus (Bath).
• Membrane-associated chromate reductase activity from Enterobacter cloacae.