Effect of (+)-dehydrofukinone on GABAA receptors and stress response in fish model
AUTOR(ES)
Garlet, Q.I., Pires, L.C., Silva, D.T., Spall, S., Gressler, L.T., Bürger, M.E., Baldisserotto, B., Heinzmann, B.M.
FONTE
Braz J Med Biol Res
DATA DE PUBLICAÇÃO
27/11/2015
RESUMO
(+)-Dehydrofukinone (DHF) is a major component of the essential oil of Nectandra grandiflora (Lauraceae), and exerts a depressant effect on the central nervous system of fish. However, the neuronal mechanism underlying DHF action remains unknown. This study aimed to investigate the action of DHF on GABAA receptors using a silver catfish (Rhamdia quelen) model. Additionally, we investigated the effect of DHF exposure on stress-induced cortisol modulation. Chemical identification was performed using gas chromatography-mass spectrometry and purity was evaluated using gas chromatography with a flame ionization detector. To an aquarium, we applied between 2.5 and 50 mg/L DHF diluted in ethanol, in combination with 42.7 mg/L diazepam. DHF within the range of 10-20 mg/L acted collaboratively in combination with diazepam, but the sedative action of DHF was reversed by 3 mg/L flumazenil. Additionally, fish exposed for 24 h to 2.5-20 mg/L DHF showed no side effects and there was sustained sedation during the first 12 h of drug exposure with 10-20 mg/L DHF. DHF pretreatment did not increase plasma cortisol levels in fish subjected to a stress protocol. Moreover, the stress-induced cortisol peak was absent following pretreatment with 20 mg/L DHF. DHF proved to be a relatively safe sedative or anesthetic, which interacts with GABAergic and cortisol pathways in fish.
Documentos Relacionados
- Photodynamic Effects of Steroid-Conjugated Fluorophores on GABAA Receptors
- Repeated neonatal handling with maternal separation permanently alters hippocampal GABAA receptors and behavioral stress responses
- Effect of sılymarın on oxıdatıve stress and lıver hıstopathology ın experımental obstructıve jaundıce model
- Frequency-dependent actions of benzodiazepines on GABAA receptors in cultured murine cerebellar granule cells.
- Carisoprodol-Mediated Modulation of GABAA Receptors: In Vitro and in Vivo Studies