Efeitos do polivinilpirrolidona-iodo (PVPI) no tratamento da peritonite bacteriana induzida em ratos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

INTRODUCTION: Controversies in the treatment of peritonitis have been taking questions about of the therapeutic value of wash with saline solution or local antiseptics. OBJECTIVES: to evaluate the effectiveness of povidone-iodine (PVPI) added to the liquid of wash of the peritoneal cavity in the reduction of the bacterial absorption and phagocytosis by hepatic splenic and pulmonary Mononuclear Phagocytic System (MPS) METHODS: It was studied 34 Wistar females rats randomly distributed in the following three groups: CONTROL (n=10), none treatment; IODINE (n=9), wash of the peritoneal cavity with 1% iodine solution; SALINE (n=15), wash of the cavity with saline solution. After anesthesia of the animals, it was made intraperitoneal infusion of a solution of 99mTc-labelled Escherichia coli containing 2,5 x1010 CFU/ml. After 40 minutes, through a median laparotomy it was made the treatment, that was, in the group CONTROL, manipulation of intestinal loops; in the group IODINE, irrigation of the peritoneal cavity with solution of warm 1% PVPI to 37,5ºC, followed by your aspiration, and in the group SALINE irrigation with saline solution heated up to 37,5ºC followed by your aspiration. After 15 minutes of the treatment, the animals were laparotomized and killed by abdominal aorta section. Samples of blood, liver, spleen and lung were obtained to radioactivity count and determined bacterial absorption index, phagocytosis index per gram of liver, spleen and lung and the remainder index in the peripheric blood. RESULTS: Of the total of bacteria infused into peritoneum, there was absorption of 0,92% (0,14% to 2,13%) in the animals of CONTROL group, 0,49% (0,18% to 0,71%) after use of topical IODINE and 0,80% (0,04% to 3,8%) after wash with saline solution. There was significant reduction of the absorption when compared the treated 4 animals with PVPI and the CONTROL group (p=0,003). Of the total of bacterias absorbed to circulatory current, the percentile of non-phagocyted bacteria in the peripheric blood was of 2,9% (1,1% to 17,7%) in the animals not treated, 15,2% (8,3% to 21,4%) in those treated with IODINE and 6,9% (0,8% to 29,7%) after wash with saline solution, with difference among the CONTROL group and IODINE group (p=0,01). There was not significant difference among the groups in relation to the hepatic, splenic and pulmonary phagocytosis. CONCLUSION: The wash of the peritoneal cavity of rats with solution containing IODINE showed to be able to reduce the absorption of bacteria for the peritoneum of rats, however it seems to interfere with the function of free phagocytic cells free for the observation of the increase of viable bacteria in the peripheric blood of those animals

ASSUNTO(S)

cavidade peritoneal decs ratos decs fagocitose decs peritonite/tratamento decs lavagem peritoneal decs

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