Efeitos da restrição proteica gestacional em hipocampo de ratos machos adultos : avaliação da estrutura dendrítica tridimensional, do comportamento e de componentes neuroquímicos = Effects of gestational protein restriction in hippocampus of adult male rats / Effects of gestational protein restriction in hippocampus of adult male rats : evaluation of three-dimensional dendritic structure of hippocampal neurons, behavior and neurochemical components

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

30/07/2012

RESUMO

Studies have demonstrated that maternal nutritional restriction during pregnancy or in early postnatal life results in hippocampus cognitive impairment and structural abnormalities in the 16-wk-old offspring. In an attempt to analyze whether gestational protein restriction might induce learning and memory impairment associated with structural changes in the hippocampus we carried out MWM test and a detailed morphometric analysis of dendritic cytoarchitecture of the hippocampus from male adult rats. In addition, we analyzed the dorsal and ventral hippocampal expression and localization of mineralo- (MR) and glucocorticoid (GR), type 1 angiotensina II receptor (AT1) and serotonin specific receptors (5HT1A and 5HT2A). By MWM we did not found significant differences between LP and NP groups, in any of the parameters analyzed, suggesting that such functions of hippocampus were not altered by gestational protein restriction. However, by applying 3-dimensional analysis of dendrites from the dorsal hippocampus, this study demonstrates that gestational protein restriction leads to decreases in total basal dendritic length and inapical intersections of CA3 pyramidal neurons. The dendritic architecture of CA1 and dentate gyros was unchanged. The current study revealed a clear dissociation between behavioral test response and hippocampal neuron changes as consequence of fetal programming. We found different patterns of dorsal and ventral expression of analyzed receptors and we suggests that reduced GR and 5HT1A and enhanced 5HT2A expression are involved in anxious behavior and that AT1 down regulation may has a protective effect. These neurochemical alterations may have important consequences for anxiety- and depressive-like behavior. Our study is not able to answer the question whether these alterations are related to in uteri underdevelopment or results from a postnatal adaptation to programmed physiology in adult life. Further time-course studies should be done to answer this question.

ASSUNTO(S)

feto - desenvolvimento glucocorticoides hipocampo fetal glucocorticoids hippocampus

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