Efeitos da geleia real sobre a resposta hematopoetica em camundongos portadores do tumor ascitico de Ehrlich

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

Alterations in the hematopoietic response are common in both human and animal tumor system. Thus, in this work we have investigated the hematopoietic response of tumor-bearing mice treated with Royal JelIy, employing the well established Ehrlich ascites tumour (EAT) experimental model. Royal Jelly (RJ) was given orally (500, 1000 and 1500 mg/kg) in a prophylactic-therapeutic manner (20 days before Ehrlich s cells inoculation and during 3, 8 or 13 days after it). Twenty-four hours after the last dose of RJ, the colony forming-unit (CFU-C) on the bone marrow and spleen was measured. Our results demonstrated that the treatment with the three doses of RJ recovery totally the myelossupression induced by the EAT, starting on the eight day after tumor inoculation. Moreover, the dose of 500 mg/kg increased the number of CFU-C in relation to normal mice. RJ treatment prevented partially, the presence of extamedullary hematopoiesis with the doses of 500 and 1000 mg/kg. Regarding to the colony stimulating activity (CSA), which was enhanced in the serum of EAT-bearing mice, it was partialIy inhibited when these mice were previously treated with 500 mg/kg of RJ for twenty-eight days. In relation to the effects of RJ in vitro, we have demonstrated that this substance increased the numbers of CFU-C, as welI as, the cellularityin the supematant of cultures treated with 100 or 500 mg/L. The study of survival showed a protection of EAT-bearing mice treated with the three doses of RJ studied, although EAT-bearing mice treated with 500 mg/kg of RJ presented higher rate of survival in relation to 1000 or 1500 mg/kg. The results obtained in this study indicate that RJ produces a modulation on the hematopoese medullar and extramedullar in EAT-bearing mice, which is probably related with the increased rate of survival in RJ treated mice

ASSUNTO(S)

hematopoese tumores asciticos

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