"Efeitos da anemia ferropriva e da estimulação tátil sobre a morfologia do nervo óptico em ratos." / "Effects of the iron-deficient anemia and the tactile Stimulation on the Morphology of the Optic Nerve"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Several works have been shown that the deficient ingestion of iron can cause alterations in the morphologic and biochemical parameters of the Central Nervous System (CNS) of the rat as well as in its behavior. Also, the iron deficient animals show reduction in the number of myelin lamellae and learning deficits. On the other hand, the tactile stimulation can reduce and/or to avoid the damages caused by the bad nutrition about CNS and behavior. However, the damages in the myelinization process have been seen also in the iron-deficient anemia and, at once that the optical nerve is formed largely by the myelinated axons, the objective of this study is to verify the effects of the anemia and tactile stimulation on the morphology of the optical nerve of Wistar males rats at the 18th, 22 nd and 32 nd days of age. The animals were divided in 2 groups: Controls © (35 mg Fe / diet Kg), Anemic (A) (4 mg Fe / diet Kg). Each group was subdivided in Stimulated (S) and No Stimulated (N). The corporal weight, haemoglobin, haematocrit, area, perimeter, minimum diameter, density of glial cells and number of vases were evaluated. The animals were anesthetized and sacrificed by heart perfusion with PBS 0,05 M in pH 7,3, following for paraformaldheyde 2% plus glutaraldheyde 1% in a phosphate buffer 0,1 M (pH 7,3). The optic nerve was dissected and submerged in solution of osmium tetroxide 1% for two hours at 40C, dehydrated in acetone with decreasing dilution degrees and included in araldite. The samples of optic nerve were guided to allow transverse cuts of their fibers. Sections sized 0,5 m were obtained and stained with toluidin blue 1% prior examinations in the light microscopy. The morphometric studies were made with a semiautomatic analyzing system of images (MiniMop). The results show that the anaemia caused differences in the corporal weight, haematocrit, haemoglobin, area, perimeter, minimum diameter, number of glial cells and vassels of the optical nerve among controls and anaemic, and that the stimulation influenced significantly on these items taking to the conclusion that it improves the damaged structures due to anaemia, but it doesn’t recover them totally.

ASSUNTO(S)

optic nerve nervo Óptico “handling” "handling" anemia anaemia

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