Efeito do tratamento com fatores hepatotróficos sobre o fígado de ratas (Wistar) com fibrose induzida experimentalmente / Effect of the treatment with hepatotrophic factors on the liver of rats (Wistar) with experimentally induced-fibrosis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The extraordinary capacity of growth of hepatic tissue can be stimulated by two processes, by the partial hepatectomy or by hepatotrophic substance supply. In this work, it was adopted the second option to treat rats with induced-fibrosis, verifying the effect of the hepatotrophic factors on the histopathological evaluation, the ratio volumetric of the collagen and the functional condition of the liver of Wistar rats with induced-fibrosis by dimetilnitrosamine. After 5 weeks of treatment, the animals had been submitted to hepatic biopsies and divided in 2 groups: Group C received 40 ml/kg of physiological solution by intraperitoneal application each 12h, during 10 consecutive days and Group F received hepatotrophic factors in same dosage and frequency of Group C. Blood collection was proceeded moments before the sacrifice and samples of the liver had been submitted to the light microscopy routine of fixation and inclusion. The histopathologic evaluation showed the presence of characteristic injuries of hepatic fibrosis, such as: regenerative nodules, biliar ducts proliferation, inflammatory infiltrated with presence of oval cells and hepatocyte megalocytes, and the animals of Group F presented reduction of the above description. The weight of the animals of group F increased 13%, the weight of the liver increased 12.2% and the weight of the liver in relation to the weight of the carcass increased 16%. The volumetric collagen density in hepatic lobe decreased 43% in group F, while in Group C it icreased 6%. The central lobular vein perivascular collagen increased in 42.7% in group C and decreased 43.4% in group F. In the portal space, the perivascular collagen increased 6.7% in group C and reduced 67.3% in group F. The serum concentration of albumen in Group C was 3,8g/l and in Group F 4,7g/l. The total protein was 6,1g/l in Group C and in Group F 7g/l. The aspartate aminotransferase AST was 148,3 U/l in 94,9 Group C and U/l in Group F, alanine aminotransferase ALT was 86,9 U/l in 44.1 Group C and U/l in Group F. We concluded the intraperitoneal hepatotrophic factors injection had reduced the histopathological signs and the collagen volumetric density, promoted better recovery of the hepatic function in Group F when compared to Group C.

ASSUNTO(S)

liver fígado collagen colágeno hepatic regeneration regeneração hepática

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