Efeito de flavonoides no eritrocito de individuos normais e portadores de anemia falciforme

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Oxygen reactive species (ROS) are constantly formed in aerobic cells, particularly in the mithocondria and erythrocytes. These ROS, under normal conditions, are removed by an efficient antioxidant system composed by antioxidant enzymes, glutathione, vitamines and microelements. However, when the ROS production is higher than the antioxidant system capacity, pathological reactions can occur characterizing the oxidative stress condition. In some pathologies, such as sickle cell anaemia, thalassemia and glucose-6-phosphate desidrogenase deficience, the oxidative stress is increased. The sickle cell anaemia is a genetic disease characterized by an increase of ROS generation, abnormal iron release and lower antioxidant activity. Several therapies have been used in order to decrease the oxidative damages of these pacients, such as the acid ascorbic and vitamine E administration. Thus, it was interesting to investigate the effect of natural antioxidant compounds, the flavonoids, in oxidative processes in the blood of sickle cell anaemia individuais and to compare the results with of normal individuais. The flavonoids are compounds widely distributed in the nature and normaly consumed by humans since they are present in great amount in the human diet; their antioxidant, antiosteoporotic, antitumor, antinflamatory and antiviral activities have been described. The effect offlavonoids (quercetin and rutin) was studied in the process of Hb oxidation, binding of Hb to celular membrane, lipid peroxidation of erythrocyte membrane and protein carbonyl groups formation in the blood of sickle anaemia and normal individuais. In general, the oxidative damage levels found in the blood of sickle individuais were higher than that found in the blood of normal individuais, differing only in the experiments of Hb oxidation. In this case, the results were similar to both Hb, perhaps because of great amount of Hb A in the blood of sickle patients. The quercetin showed higher antioxidant effect in ali experiments. The rutin behaved as pro-oxidant in the Hb oxidation, when rutin is used in concentration lower than 150 11M, however it showed protective effect in higher concentrations. Supported by these results, we can suggest beneficial effects of flavonoids, mainly of quercetin, in the protection against oxidative damage of sickle anaemia individuais blood

ASSUNTO(S)

eritrocitos anemia falciforme flavonoides hemoglobina

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