Efeito de diferentes concentrações do alendronato sódico sobre a viabilidade e proliferação de diferentes tipos celulares em cultura / Effect of different concentrations of alendronate on the viability and proliferation of different cell types

AUTOR(ES)

Mariana Aparecida Brozoski

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

28/04/2011

RESUMO

Bisphosphonates have been therapeutically used for the management of lytic bone diseases. Their use has been increased nowadays and besides that associated adverse effects have been amplified. Jaw osteonecrosis induced by this drug is perhaps the most important complication because of the great morbidity and difficulty to deal with. Until now the physiopathology of osteonecrosis remains unclear and the treatment that should be established is uncertain. This study aimed to evaluate the effect of sodium alendronate a bisphosphonate used for the treatment of osteoporosis on the viability and proliferation of osteoblasts and fibroblasts in culture. Osteoblast-simile from the lineage OSTEO1 and a human oral mucosa fibroblasts from the lineage FMM1 were used. After being subjected to tests with concentrations of sodic alendronate ranging from 10-8M to 10-2M fibroblasts showed a significant decrease in cell viability at the concentration of 10-2M (p <0.01). Osteoblasts showed that the cell viability in the control group was significantly higher than all other groups, the group treated with alendronate at a concentration of 10-4M had similar cell viability with all groups except the group of 10-2M concentration and the cell viability of other groups was similar between groups (p <0.01). The concentrations of alendronate greater than 10-5M prevented the proliferation of osteoblasts. It was possible to conclude that alendronate is cytotoxic to osteoblast-símile cells and fibroblasts in culture due to its concentration. The fibroblasts are less sensitive to higher concentrations of alendronate than osteoblasts.

ASSUNTO(S)

alendronate alendronato jaws mandíbula maxila osteonecrose osteonecrosis

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