Efeito da tiroxina e do hormônio folículo estimulante no desenvolvimento in vitro de folículos pré-antrais ovinos / Effects of thyroxin and follicle stimulating hormone on the in vitro development of ovine preantral follicles

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

22/02/2010

RESUMO

This experiment has been developed in order to verify the thyroxine effect in the presence or absence of follicle stimulating hormone (FSH) on survival and growth of sheep preantral follicles. Fragments of ovarian tissue were grown for 1 or 7 days in a situation with a minimum essential (α-MEM +) alone or containing thyroxine (10, 20, 50 g/mL) in the presence or absence of rFSH (50 ng/mL). The ovarian tissue non-grown (fresh control) and grown in different treatments were processed for analysis using the classical histology technique. When the days of culture were compared to the non-cultivated control, on the first day was found that the treatment with α-MEM+, FSH, T20 and T50 showed follicular survival percentages similar to control. When the treatments have been compared between each other within the growing season was identified that only on the 7th day, the treatment containing T50 presents a percentage of normal follicles significantly superior to the treatment with T10F. Related to the follicular diameter, the follicle in culture has not grown, since all treatments have had a similar behavior when controlling non-grown and α- MEM+, except the T10 treatment. When comparing the treatments within each growing season, it has been observed that on the first day, treatments T50, T10F and T50F have showed a follicular diameter greater when compared to T10 treatment. Similar to what happenedto follicular growth, oocitário growth has not grown either. For oocitário diameter, on the first day, there was a reduction for T10 and T20F treatments when compared to non-grown. On the 7th day of grown, FSH, T10F and T50F treatments have reduced oocitário diameter, with a progression of culture from the first day to the 7th one and even when compared to control non-grown and α-MEM+. It was concluded that high T4 concentrations (50 g / ml) have provided promising about the survival and follicular development in vitro. The association of T4 with FSH, have not presented positive results in the survival and follicular development in vitro. Although it is known the thyroxine action on ovarian physiology, there is a need to verify its importance for the in vitro development of preantral sheep follicles.

ASSUNTO(S)

reproducao animal folículos pré-antrais tiroxina fsh ovinos preantral follicles thyroxin fsh sheep

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