Efeito da rutina em eritrocitos de ratos : estudo in vitro e in vivo

AUTOR(ES)

Daniela Garcia de Oliveira

DATA DE PUBLICAÇÃO

2001

RESUMO

Many studies have investigated the ability of different compounds to protect biological systems from oxidative damage. In particular, flavonoids obtained from plants have been shown to inhibit reactions, whieh involve the produetion of radicais sueh as hidroxyl radical, and superoxide anion, as well as peroxyl and alkoxyl radicais. The antioxidant action of flavonoids may also be related to their ability to ehelate metal ions, which in tum eould eontribute to the inhibition of lipid peroxidation of membrane erythroeyte. In the present investigation, the effeet of rutin "in vitro" and " in vivo" on hemoglobin (Hb) oxidation and erythroeyte membrane peroxidation was examined in blood of rats treated with phenylhydrazine (PHZ) that is able to generate phenyl radicais and superoxide anion thereby an oxidant drug. Experiments "in vitro" demonstrated pro-oxidant behavior of rutin in the presence of PHZ as shown by measurements of reactive thiol groups in Hb, GSH, Hb oxidation, binding of Hb to erythroeyte membanes, lipid peroxidation and isotonie hemolysis. Reverse-phase HPLC (RP-HPLC) showed that incubating rutin with PHZ resulted in two products (pr-1 and pr-2) , both of which were more active as oxidants than PHZ when tested with Hb, increasing metHb production. In contrast to the results "in vitro", experiments "in vivo" showed that rutin protected the system against oxidative damage by PHZ, using as indicators Hb oxidation, reactive thiol groups in Hb, GSH, binding of Hb to membrane, Heinz body formation, lipid peroxidation and osmotie resistance. These results were obtained with rat blood treated with intraperitoneal administration of rutin for 10 days. RP-HPLC of plasma from these animais showed the presence of a peak with retention time different from that obtained for rutin suggesting to be a metabolite of the fIavonoid. Histological examination of blood samples and oxidation experiments of animals treated with 1,5 mom rutin for 10 days showed that the extent of erythrocyte damage caused by 60 mg PHZI Kg of animal was lower than those observed for non-rutin treated animais

ASSUNTO(S)

flavonoides agentes oxidantes stress (fisiologia)

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