EFEITO AGUDO E AVALIAÇÃO HISTOPATOLÓGICA DE LARVAS DO PEIXE-REI MARINHO Odontesthes argentinensis EXPOSTAS À FRAÇÃO SOLÚVEL DO PETRÓLEO, DIESEL E GASOLINA EM ÁGUA

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The hydrocarbon of petroleum is currently considered as one of the main pollutants of water resources and cause recognized toxicity to aquatic biota. However, there are few studies regarding the toxicity of these compounds on the early stages of development in Brazilian fish. The objective of this study was to evaluate the median lethal concentration (LC50-96h) and the histopathological effects of water-soluble fraction (WSF) of oil, diesel and gasoline, on larvae of marine pejerrey Odontesthes argentinensis. During the experiments the concentrations tested were: Brazilian crude oil (5%, 10%, 25%, 50%, 75%, and 100% of WSF), diesel (1%, 2%, 4%, 8%, 16%, 32%, e 64% of WSF) and gasoline (1%, 2.5%, 5%, 10%, and 20% of WSF) plus a control for each pollutant. All treatments were performed with three replicates and 30 larvae. After the toxicity tests (96 h), three larvae of each concentration were collected for histological evaluation. The petroleum presented a median lethal concentration (LC50-96h) equal to 70.68% (65.73-76.01), while diesel and gasoline presented the LC50-96h values of 13.46% (10.19-17.79) and 5.48% (4.85-6.20), respectively. The histological evaluation of O. argentinensis larvae exposed to WSF of petroleum, diesel and gasoline showed lesions in the gills, pseudobranchs, esophagus and liver. The gills, pseudobranchs and esophagus showed epithelial hyperplasia, while the liver presented dilation of hepatic sinusoids, hepatocitomegaly, hepatocytes bi-nucleated and nuclear degeneration of hepatocytes, as well as piknotic nuclei. The present investigation demonstrated that diesel and gasoline are at least five times more toxic than Brazilian petroleum, however all toxicants induced to moderate histopathological injuries in pejerrey larvae.

ASSUNTO(S)

recursos pesqueiros histopatologia hidrocarboneto aquicultura petróleo zootecnia peixe-rei

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