EFEITO ADJUVANTE DA Propionibacterium acnes E DE SEU COMPONENTE POLISSACARÍDICO SOLÚVEL PURIFICADO SOBRE A MIGRAÇÃO E ATIVIDADE CITOTÓXICA DE CÉLULAS DO EXSUDATO PERITONEAL DE CAMUNDONGOS C57Bl/6. / Modulatory effect of killed-Propionibacterium acnes and its purified soluble polysaccharide on peritoneal exudate cells from C57Bl/6 mice: major NKT cell recruitment and increased cytotoxicity

AUTOR(ES)

Renata Zeigler Ananias

DATA DE PUBLICAÇÃO

2006

RESUMO

In the present work we analyzed the effect of heat-killed-Propionibacterium acnes or its purified soluble polysaccharide (PS) injected by intraperitoneal route in C57Bl/6 mice on peritoneal exudate cells (PEC), aiming at their distribution and cytotoxicity. Both treatments induced an increase in macrophages, immature dendritic, and NKT cells. The bacterium caused a strong increase in the NK1.1+CD3+CD4-CD8- subpopulation and a decrease of B1a lymphocytes, while PS induced an increase in the NK1.1CD3+CD8+ subpopulation and a marked increase of B1a cells in the peritoneal cavity. To verify P. acnes and PS adjuvant effect on PEC cytotoxic properties we evaluated in vitro their effect on murine B16F10 melanoma cells. The effector cells from the bacteria and PS-treated groups lysed melanoma cells in co-cultures with PEC. The cytotoxicity seemed to be related to NO production, since it was completely abrogated in PEC from iNOS-/- mice. Cytotoxicity from P. acnes-treated mice also depended on IFN- and TNF- since lysis decreased with PEC from IFN- knockout mice or by adding anti-TNF- to the co-cultures. Tumoricidal activity in PEC from P. acnes-treated group is mediated by macrophages and NKT; this activity in PEC from PS-treated mice is played only by macrophages. Both treatments also increased IL-4 and IFN- synthesis by NKT cells. We show that P. acnes act mainly in recruiting and activating NKT cells in PEC, which also play an important tumoricidal activity independent of IFN- . An important mechanism that mediated bacterial effects is the NO release that seems to be predominantly stimulated by the polysaccharide present in the bacterial cell wall.

ASSUNTO(S)

1. propionibacterium acnes. 2. imunomodulação. 3. células do exsudato peritoneal. 4. função citotóxica. microbiologia

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