Easy identification of cDNA clones.
AUTOR(ES)
Rüther, U
RESUMO
A set of six cloning vectors, pUR 278, 288, 289, 290, 291, 292 is presented. These vectors have the cloning sites, BamHI, SalI, PstI, XbaI and HindIII, in all frames at the 3' end of the lacZ gene. Insertion of cDNA in the proper cloning sites leads to a fusion protein of active beta-galactosidase and the peptide encoded by the cDNA. A simple immunoenzymatic assay can be used to identify clones in such a cDNA library.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=555360Documentos Relacionados
- Construction and identification of mouse amelogenin cDNA clones.
- Isolation and characterization of human defensin cDNA clones.
- Isolation and analysis of chick homeobox cDNA clones.
- Construction of infectious potato spindle tuber viroid cDNA clones.
- Isolation and characterization of Borna disease agent cDNA clones.