Double-Layer Plaque Assay for Quantification of Enteroviruses
AUTOR(ES)
Mocé-Llivina, Laura
FONTE
American Society for Microbiology
RESUMO
We describe here a double-layer plaque assay for the quantification of enteroviruses, combining a monolayer plaque assay and a suspended-cell plaque assay. The double-layer assay provides significantly greater counts than other methods of virus quantification of both suspensions of pure culture viruses and naturally occurring viruses. The counts obtained by this method are approximately one order of magnitude greater than those obtained with the more commonly used method, the monolayer plaque assay. We conclude that the methods available for quantifying viruses rank in efficiency as follows: double-layer plaque assay ≥ suspended-cell plaque assay > counting cytopathogenic virus adsorbed to cellulose nitrate membrane filters ≥ most probable number of cytopathogenic units > monolayer plaque assay. Moreover, the double-layer plaque assay allows the use of two different cell lines in the two layers. Using the human colonic carcinoma cell line CaCo2 facilitates the recovery of a greater number and diversity of naturally occurring enteroviruses in water than the monolayer agar method. In addition, the pretreatment of cells with 5-iodo-2′-deoxyuridine (IDU) prior to the quantification of enteroviruses by the double-layer plaque assay provides significantly higher recoveries than the use of IDU does with the other methods of quantification.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=404442Documentos Relacionados
- Nitinol double-layer stent versus closed single-layer stent: a systematic review
- LINEAR STABILITY ANALYSIS AND CFD SIMULATION OF DOUBLE-LAYER RAYLEIGH-BÉNARD CONVECTION
- Estudo das propriedades estruturais e eletrônicas de bundles de nanotubos de BN dupla camada sob pressão
- Adhesion of hard spheres under the influence of double-layer, van der Waals, and gravitational potentials at a solid/liquid interface.
- Plaque Assay for Ebola Virus