DNase I hypersensitive sites in Drosophila chromatin occur at the 5' ends of regions of transcription.

AUTOR(ES)

Keene, M A

RESUMO

By using a map of the unique region of DNA encoding the fur small heat-shock proteins of Drosophila melanogaster (hsp 22, hsp 23, hsp 26, and hsp 28), and a simple mapping technique, the positions of the DNase I hypersensitive sites of chromatin in the vicinity of these genes have now been determined. The major chromatin-specific sites occur at the 5' ends of each of the four heat-shock protein genes in embryo nuclei. These genes are not active in the nuclei analyzed but can be quickly induced in these cells by the heat-shock stimulus. The chromatin structure indicated by DNase I hypersensitivity may be a necessary factor in the general mechanism of gene activation.

Documentos Relacionados

• Multiple transcription start sites, DNase I-hypersensitive sites, and an opposite-strand exon in the 5' region of the CHO dhfr gene.
• DNase I hypersensitive sites of the chromatin for Drosophila melanogaster ribosomal protein 49 gene.
• DNase I-hypersensitive sites in the chromatin of rat growth hormone gene locus and enhancer activity of regions with these sites.
• Constitutive and anaerobically induced DNase-I-hypersensitive sites in the 5′ region of the maize Adh1 gene
• DNase I-hypersensitive sites in the chromatin of immunoglobulin kappa light chain genes.