DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase
AUTOR(ES)
Roberts, B. Tibor
FONTE
The National Academy of Sciences
RESUMO
CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA replication has not yet been addressed. Here we show that antibodies to the Xenopus laevis homolog, xCdc7, interfere with DNA replication in vivo in developing embryos and in vitro in cycling egg extracts. We also demonstrate cell cycle-dependent association of xCdc7 with the Mcm complex, which binds to replication origins and also is required for DNA synthesis. Taken together, these data indicate that the function of xCdc7 is conserved from fungi to vertebrates. xCdc7 protein accumulates after stimulation of resting oocytes with progesterone, suggesting a molecular explanation for previous observations that the development of the capacity for DNA replication requires protein synthesis late in meiosis I.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=15849Documentos Relacionados
- A Xenopus Dbf4 homolog is required for Cdc7 chromatin binding and DNA replication
- DNA metabolism gene CDC7 from yeast encodes a serine (threonine) protein kinase.
- Transcriptional analysis of the CDC7 protein kinase gene of Saccharomyces cerevisiae.
- The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity.
- Molecular Genetic Studies of the Cdc7 Protein Kinase and Induced Mutagenesis in Yeast