DNA replication in Physarum polycephalum: characterization of replication products in vivo.
AUTOR(ES)
Funderud, S
RESUMO
Synchronous plasmodia of Physarum polycephalum in DNA synthesis were pulse-labelled with [oH]- thymidine for time periods of 15 seconds up to 9 minutes, or given a 30 seconds pulse followed by chase periods of 9 minutes up to 6 hours. Sedimentation analysis in alkaline sucrose gradients revealed at least five species of single stranded DNA14 molecules in the pulse experiments. Co-sedimentation of [14C]-labelled phage-DNA gave relative S-values of 5-7, 13-15, 23-25, 30 and 33-35 for these DNA molecules, all of which can be chased into DNA of higher molecular weight.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=344389Documentos Relacionados
- DNA replication in Physarum polycephalum: characterization of DNA replication products made in vivo in the presence of cycloheximide in strains sensitive and resistant to cycloheximide.
- DNA replication in Physarum polycephalum: bidirectional replication of DNA within replicons.
- DNA replication in Physarum polycephalum: UV photolysis of maturing 5-bromo-deoxyuridine substituted DNA.
- Starvation phase of Physarum polycephalum: characterization of transfer ribonucleic acid.
- Temperature-sensitive mutants of Physarum polycephalum: viability, growth, and nuclear replication.